Unheated greenhouse: 5–10°F warmer than outside at night.
Vent on sunny days—temps can spike to 100°F+.
Use a min/max thermometer to track actual conditions.
Your Situation
Losses at 40°F suggest seedlings weren't hardened off. Next batch: start hardening 2 weeks before planned transplant date. Keep a log of species and temps—patterns will emerge.
Respect the transition. Hardened seedlings handle cold; soft ones don't.